The Effect of Pegagan (Centella asiatica (L.) Urban) as Material Antifertility to GPT (Glutamic Pyruvate Transaminase) and GOT (Glutamic Oxaloacetic Transaminase) Concentration and Its Histological Profile of Female Mice (Mus musculus)

Abstract:

One plant that allegedly has the potential as antifertility material is pegagan (Centella asiatica (L.) Urban). Based on previous research, high dose of pegagan , 275 mg / kg BW is effective to reduce the number of primary, secondary and tertiary follicles.

The follicular development was not even discovered until the stage of de Graff. Characteristics of an ideal contraceptive is safe, efficient, cheap, easily obtained, reversible and a little of side effects on other organs including liver. The purpose of this study was to determine whether pegagan is potential for safe contraception and does not cause toxic effects on the liver because this organ plays an important role in metabolic processes such as modifying drugs and toxins become inactive or water soluble. The design used in this study were completely randomized design with four treatments in four doses, ie 125 mg / kg, 200 mg / kg, 275 mg / kg BW and control, each  treatment consisting of six replicates. Dependent variables observed in this study is the level of GPT (glutamate pyruvate transaminase) and GOT (glutamic oxaloacetic transaminase) and profile of liver histological mice. GPT and GOT levels measured were analyzed by analysis of single variant of 0.05. If the F calculated> F table it is followed by LSD 0.05. Histological picture of the liver include central venous dilation and inflammation and damage to the structure of liver cells in the scoring and then performed Kruskal Wallis. Statistical analysis showed that the leaf extract of pegagan as a material antifertility not provide significant effect on serum GPT and GOT enzyme in the liver of mice, whereas the histology results did not reveal any visible damage to liver cells in each treatment or dose given unless the treatment dose 200 mg / kg BW seems there is little central venous dilation and degeneration of liver cells in several fields of view but after statistical analysis did not provide significant differences between treatments. From the above results concluded that pegagan extract as an antifertility substances until the dose of 275 mg / kg is safe and will not cause damage to the liver.

Keywords: pegagan extract (Centella asiatica (L) Urban), levels of GPT, GOT, liver histology, female mice (Mus musculus)

By: Bayyinatul Muchtaromah
Department of Biology, Faculty of Science and Technology

University of Islamic State Maulana Malik Ibrahim Malang

Jl. Gajayana 50 Malang, East Java, Indonesia Phone / fax : 0341 558933

Mobile phone: 0811369317 Email: bayyinatul_uin@yahoo.co.id